[Objective] To establish a pipeline for unknown transcriptional start site(TSS) identification without radioactivity,we used genetic fragment analysis system and replenished two steps regarding prediction and evaluation.[Methods] We used unknown TSSs of Gro EL genes from M.xanthus as a case.Firstly,we predicted the potential TSSs through bioinformatics databases.According to the prediction,we designed and synthesized fluorescence labeled primers to carry out the reverse transcription reactions.Further,we to...
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