【摘要】 目的分别使用昆明小鼠急性经口毒性试验、秀丽隐杆线虫毒性试验及CHL体外细胞毒性试验研究纳米铜的急性毒性作用。方法小鼠急性经口毒性试验采用霍恩氏法;线虫急性毒性试验对秀丽隐杆线虫L4期幼虫进行24 h染毒;体外细胞毒性试验采用CHL细胞中性红染色法。结果纳米铜小鼠急性经口毒性试验雌性小鼠经口半数致死量LD50为14.0 mg/kg·bw,雄性小鼠经口半数致死量LD50为8.8 mg/kg·bw;线虫急性毒性试验显示,随着纳米铜染毒剂量的增加,线虫死亡率呈上升趋势,纳米铜对线虫IC50为12.6μg/ml;细胞毒性试验显示,随着纳米铜染毒剂量的增加,CHL细胞存活率呈下降趋势,纳米铜对CHL细胞IC50为2.33μg/ml。结论根据化学品急性经口毒性试验方法(GB/T 21603-2008)的分级标准判定,纳米铜小鼠急性经口毒性属高毒;12.9和30μg/ml纳米铜对线虫有毒性作用;大于3.75μg/ml纳米铜细胞毒性为4级。更多还原

【Abstract】 Objective To study the acute toxicity of copper nanoparticles through acute oral toxicity test of Kunming mice,acute toxicity test of Caenorhabditis elegans and cytotoxicity test of CHL cell in vitro. Methods Horns method was used in the acute mice oral toxicity test of copper nanoparticles. The acute toxicity of copper nanoparticles on L4 stage larvae was tested at 24 h after different doses of copper nanoparticles exposure. Neutral red uptake assay was used in cytotoxicity test of copper nanoparticles. Results The LD50 of copper nanoparticle to male mice was 8. 8 mg/kg·bw. Copper nanoparticles can cause a dose dependent inhibition on the growth of L4 stage larvae.The IC50 of copper nanoparticles to Caenorhabditis elegans was 12. 6 μg/ml. The cytotoxicity test showed that copper nanoparticles can cause a dose dependent inhibition on the cell survival rate. The IC50 of copper nanoparticles to CHL cell was 2. 33 μg/ml. Conclusion According to "Test method of acute oral toxicity for chemicals( GB/T 21603-2008) ",copper nanoparticles was classified as high-toxic to Kunming mice. At doses of 12. 9 μg/ml and 30 μg/ml,copper nanoparticles has a toxic effect on Caenorhabditis elegans. At doses greater than3. 75 μg/ml,copper nanoparticles can cause a high-toxic effect on CHL cell.更多还原