【摘要】 背景:Faecalibacterium prausnitzii(Fp)数量在炎症性肠病(IBD)患者中特异性减少,既往研究显示Fp上清液在实验性结肠炎中具有明显抗炎效应。目的:探讨Fp上清液对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎模型单核-巨噬细胞和结肠炎症的影响。方法:30只雄性C57BL/6J小鼠随机分为对照组、炎症组和Fp治疗组,后两组连续7 d饮用含4.5%DSS的蒸馏水以建立结肠炎模型,Fp治疗组在造模同时予Fp上清液灌胃。观察各组小鼠体质量变化、排便情况和结肠组织病理学改变,以流式细胞术检测脾脏和结肠固有层不同表型单核细胞和巨噬细胞以及外周血细胞因子水平。结果:Fp治疗组小鼠体质量下降、结肠缩短、结肠组织病理学改变均轻于炎症组(P<0.05),脾脏和结肠固有层总单核细胞和促炎单核细胞百分率、结肠固有层M1型巨噬细胞百分率均显著低于炎症组(P<0.05),结肠固有层M2型巨噬细胞百分率则显著高于炎症组(P<0.05),外周血白细胞介素(IL)-10、IL-4水平显著高于炎症组(P<0.05),IL-6、干扰素(IFN)-γ、趋化因子CCL2水平显著低于炎症组(P<0.05)。结论:Fp上清液可通过减少促炎单核细胞数量、诱导肠道炎症部位巨噬细胞向M2型极化以及促进抗炎细胞因子分泌、抑制促炎细胞因子和趋化因子分泌等途径在小鼠结肠炎中发挥抗炎作用。更多还原

【Abstract】 Background:The abundance of Faecalibacterium prausnitzii(Fp) is reduced in patients with inflammatory bowel disease(IBD).Previous studies demonstrated that the supernatant of Fp exerts an obvious anti-inflammatory effect in experimental colitis.Aims:To investigate the effect of Fp supernatant on monocytes/macrophages and colonic inflammation in dextran sulfate sodium(DSS)-induced colitis in mice.Methods:Thirty male C57 BL/6 J mice were randomly divided into control group,colitis group and Fp treatment group.Acute colitis was induced by drinking 4.5% DSS in distilled water for seven days in colitis and Fp treatment groups.Mice in Fp treatment group were also given gastric infusion of Fp supernatant during the process of colitis induction.Weight loss,defecation and histopathological damage of colon tissue were observed.The percentages of monocytes and macrophages of different phenotypes in spleen and colonic lamina propria,as well as the serum levels of a series of inflammatory cytokines were detected by flow cytometry.Results:Compared with colitis group,Fp treatment showed an ameliorating effect on weight loss,shortening of colon length and histopathological damage(P < 0.05).The percentages of total amount of monocytes and proinflammatory monocytes in spleen and colonic lamina propria,and the percentage of M1 macrophages in colonic lamina propria in Fp treatment group were significantly lower than those in colitis group;the percentage of M2 macrophages in colonic lamina propria in Fp treatment group was significantly higher than that in colitis group(P all < 0.05).The serum levels of interleukin(IL)-10 and IL-4 in Fp treatment group were significantly higher than those in colitis group,and the levels of IL-6,interferon(IFN)-γ and chemokine CCL2 were significantly lower than those in colitis group(P all < 0.05).Conclusions:Fpsupernatant may exert anti-inflammatory effect in experimental colitis in mice via reducing proinflammatory monocytes,inducing M2 polarization of macrophages in inflamed colon,promoting secretion of anti-inflammatory cytokines,and inhibiting secretion of proinflammatory cytokines and chemokines.更多还原