【摘要】 目的:通过高脂膳食诱导肥胖小鼠模型,观察不同强度急性运动对肥胖小鼠PGC-1α及其下游因子的转录与翻译水平的影响,进一步探讨PGC-1α信号在运动减控体重中的生理机制。方法:C57BL/6J雄性小鼠经高脂膳食诱导建模成功后,随机分为安静对照组(C组)、小强度(SE组)、中强度(ME组)、大强度有氧运动组(HE组)。后3组进行不同强度的一次性跑台训练,运动后即刻处死。采用ELISA测定血清TNF-α、IRISIN、MCAD、CPT-1含量;实时荧光定量PCR测定骨骼肌PGC-1α、PPARγ、PPARβ、TNF-α及FNDC5 m RNA相对表达量。结果:与C组比较,SE组小鼠血清MCAD显著性提升(P<0.05);ME和HE组TNF-α显著性下降(P<0.05;P<0.01);CPT-1与Irisin4组间差异不具有显著性(P>0.05);与C组相比,SE、ME及HE组骨骼肌PGC-1αm RNA相对表达量显著性提升(P<0.01);ME与HE组PPARγm RNA显著性增加(P<0.05;P<0.01);ME与HE组PPARβm RNA显著性增加(P<0.01;P<0.05);3个运动组中TNF-αm RNA相对表达量显著性降低(P<0.01);ME与HE组FNDC5 m RNA相对表达量显著性增加(P<0.05)。骨骼肌PGC-1αm RNA与FNDC5 m RNA相对表达量呈显著性正相关(P<0.01);骨骼肌FNDC5 m RNA相对表达量与血清Irisin含量相关性较低(P>0.05);骨骼肌PGC-1αm RNA相对表达量与血清TNF-α含量显著性正相关(P<0.05)。结论:急性有氧运动可以显著增强PGC-1α及其下游因子的转录与翻译水平;小鼠骨骼肌氧化代谢和炎症反应之间可能存在相互拮抗的肌肉分子机制。更多还原

【Abstract】 Objective :To observe the effects of acute aerobic exercise with various intensities on the transcription and translation levels of PGC-1α and its downstream factors in obese mice through the high-fat diet induced mice obesity models,with an aim to further explore the physiological mechanism of PGC-1α signal in exercise for weight loss. Methods: C57BL/6J male mice were randomly divided into control group(group C),low intensity group(group SE),moderate intensity group(group ME),and high intensity group(group HE)after successful modeling induced by high-fat diet. The latter 3 groups were given one-time treadmill training at different intensities and were sacrificed immediately after exercise. Serum TNF-α,IRISIN,MCAD and CPT-1contents were determined by ELISA;and relative expression quantities of PGC-1α and its downstream factors PPARγ,PPARβ,TNF-α and FNDC5 m RNA in skeletal muscle were detected by means of q RT-PCR. Results: Compared with group C,the serum MCAD value in group SE was significantly improved(P<0.05);TNF-α in group ME and HE was remarkably decreased(P<0.05;P<0.01);while differences in CPT-1 and Irisin among four groups showed no statistical significance(P>0.05). Compared with group C,relative expression quantities of PGC-1αm RNA in skeletal muscle in groups SE,ME and HE were notably enhanced(P<0.01);those of PPARγ m RNA in groups ME and HE were markedly up-regulated(P<0.05;P<0.01);those of PPARβ m RNA in groups ME and HE were distinctly improved(P<0.01;P<0.05);those of TNF-α m RNA in three exercise groups were down-regulated apparently(P<0.01);and those of FNDC5 m RNA in groups ME and HE were outstandingly elevated(P<0.05). Relative expression quantity of PGC-1α m RNA in skeletal muscle showed significantly positive correlation with that of FNDC5 m RNA(P<0.01);that of FNDC5 m RNA had low correlation with serum Irisin content(P>0.05);and that of PGC-1α m RNA in skeletal muscle showed remarkably positivecorrelation with serum TNF-α content(P<0.05). Conclusion: Acute aerobic exercise can significantly enhance the transcription and translation levels of PGC-1α and its downstream factors.There may be an antagonistic muscular-molecular mechanism between the oxidative metabolism and the inflammatory response of skeletal muscle in mice.更多还原