【摘要】 采用均匀设计实验方法建立5L罐发酵生产L-精氨酸-¹⁵N₄的优化调控模型。在菌体生长24h进入产酸阶段,控制转速820r/min,pH7.8,温度30℃,通气2.5L/min,流加总量60%的葡萄糖,理论产酸最高可达到22.49g/L,验证实验产酸19.69g/L,达到理论高值的88%。利用¹⁵N丰度99.68%的尿素和丰度99.43%的硫酸铵发酵生产得到L-精氨酸-¹⁵N₄的产品丰度为98.7%,化学纯度为98.5%,丰度下降<1%。可以满足批量生产的要求。更多还原

【Abstract】 Using uniform design,the optimal control model had been established for the fermentation of L-Arginine-¹⁵N₄ in 5 Lfermentation tank.L-arginine was produced when the cell grew about 24 h.The theoretical yield can up to 22.49g/L when production condition controlled in 820r/min,pH 7.8,temperature in 30 ℃,the ventilation2.5L/min and fed-batch 60% glucose.While the actual yield was 88% of theoretical yield and 19.69g/L of L-Arginine,which was verified.L-Arginine-¹⁵N₄ was produced by the abundance 99.68% of urea and 99.43% of ammonium sulphate,which ¹⁵N isotopic abundance was up to 98.7%,the purity was 98.5%.The ¹⁵N isotope abundance decreased less than 1%.The process was satisfied the request of batch production.更多还原