【摘要】 目的研究过敏性哮喘中DC与CD4~+T淋巴细胞的相互作用,探讨过敏性哮喘中DC在体内是否可以直接趋化CD4~+T细胞,从而为哮喘治疗提供新的方向。方法分选C57BL/6哮喘模型小鼠肺部DC注射到小鼠后背气泡中,细胞注射12 h后,流式细胞仪检测气泡内CD4~+T淋巴细胞数目。结果与仅注射PBS的对照相比,注射DC组趋化至气泡内的CD4~+T细胞显著上升。结论本研究表明过敏性哮喘中DC在体内可以直接趋化CD4~+T细胞。更多还原

【Abstract】 Objective:To study the direct effect of dendritic cells(DC) on CD4~+T lymphocytes in allergic asthma,and to explore whether DC can directly induce CD4~+T lymphocytes in asthmatic mice,we performed this research. This study will provide a new direction for the treatment of asthma. Methods:Pulmonary DCs were sorted from the C57 BL/6asthmatic mice,and injected into the dorsal air pouches of the mice. The number of CD4~+T lymphocytes in the air pouches was detected by flow cytometry after 12 hours of cell injection. Results:Compared with PBS control,the chemotactic CD4~+T lymphocytes increased significantly after the DC injection. Conclusion:This study indicates that lung DCs in asthmatic mice can directly induce CD4~+T lymphocytes in vivo.更多还原