【摘要】 为观察雪灵芝粗多糖(Arenaria kansuensis crude polysaccharide,AKCP)对体外培养的小鼠脾淋巴细胞、NK细胞和腹腔巨噬细胞增殖与功能的影响。以不同浓度AKCP作用于体外培养的上述细胞48 h,采用中性红吞噬实验及NO释放实验检测巨噬细胞功能,MTT法检测脾淋巴细胞增殖及NK细胞杀伤活性,流式细胞术检测脾淋巴细胞CD3~+、CD4~+、CD8~+亚群,ELISA法检测脾细胞培养上清中IL-2和IFN-γ水平。结果显示,AKCP各浓度组小鼠腹腔巨噬细胞的吞噬活性和NO释放量、脾淋巴细胞刺激指数及培养上清中IFN-γ水平、NK细胞杀伤活性均高于空白对照组(P<0.05);AKCP中浓度组脾淋巴细胞CD3~+、CD4~+亚群及培养上清中IL-2水平也明显升高(P<0.05)。提示AKCP对小鼠免疫细胞的增殖与功能具有体外激活作用。更多还原

【Abstract】 To investigate the effects of crude polysaccharide from Arenaria kansuensis( AKCP) on the proliferation and function of murine immunocyte in vitro. After mice peritoneal macrophages and spleen lymphocytes were cultured in vitro and be treated by different concentrations of AKCP for 48 hours,the lymphocyte proliferation and NK killing activity were detected by MTT method,the CD3~+/CD4~+/CD8~+subsets in spleen lymphocytes were analyzed by FCM,and the levels of IL-2 and IFN-γ in spleen cell culture supernatant were tested by ELISA. The results showed that after high,middle and low concentrations AKCP treatment,the phagocytic and NO secreting activities of peritoneal macrophages,the proliferation index as well as IFN-γ secretion level of lymphocytes,and the NK killing activity were significantly higher than those in control group( P < 0. 05). In middle AKCP group,the CD3~+,CD4~+subset in spleen lymphocytes,the level of IL-2 secreted by lymphocytes was obviously increased( P < 0. 05). These results collectively indicated that AKCP had abilities to activate the proliferation and function of murine immunocyte in vitro.更多还原