【摘要】 分离纯化人工栽培的六妹羊肚菌子实体多糖,对其结构和抗氧化活性进行研究。采用水提醇沉法提取六妹羊肚菌子实体多糖（MSP）,采用DE-52纤维素柱和Sephadex G-100进行多糖的分离纯化,借助HPGPC和HPLC测定多糖分子量及单糖组成。通过DPPH自由基、羟自由基和超氧阴离子自由基的清除作用,评估其体外抗氧化活性。构建H₂O₂介导的氧化压力损伤的PC12细胞模型,评估其基于抗氧化的神经保护作用。结果显示,六妹羊肚菌水溶性多糖平均分子量为287 588 Da,单糖组成为甘露糖,葡萄糖和半乳糖,占比约为9∶1∶6。六妹羊肚菌多糖具有优良的自由基清除活性,同时,能够通过重塑SOD、CAT、GSH-Px等抗氧化酶系活性,缓解H₂O₂诱发的氧化压力的细胞损伤,抑制PC12细胞凋亡。涉及通路为Bax/Bcl及Caspase。六妹羊肚菌水溶性多糖具有优良的抗氧化活性,表现出一定的神经保护作用。更多还原

【Abstract】 This article mainly studied the extraction,purification,structure and antioxidant effects of a polysaccharide（MSP） purified from the fruiting bodies of Morchella sextelata M. Kuo. The polysaccharides of M. sextelata were extracted with boiling water and purified by DE-52 column and Sephadex G-100 column. The molecular weight,monosaccharide composition were analyzed by HPGPC and HPLC. In order to investigate the neuroprotective role of MSP,the antioxidant activity of MSP against the H₂O₂-induced cytotoxicity and the underlying preventative mechanisms in rat pheochromocytoma（ PC12） cells were illustrated. It was shown that the molecular weight of MSP was 287 588 Da（287 588 Da）,the monosaccharide composition of MSPwasmainly composed of mannose,glucose and galactose,with a ratio near 9∶ 1∶6. MSP can considerably enhance the viability of PC12 cells exposure to H₂O₂ and increase the activities of antioxidant enzyme like SOD,CAT and GSH-Px. It inhibited cell apoptosis via reducing caspase-3 activation and down-regulation Bax/Bcl-2 ratio. Accordingly,MSP can be used as a promising neuroprotective compound for nervous diseases treatment.更多还原