【摘要】 以普通市售马铃薯淀粉为原材料,在常温下利用W/O乳化方法制备了交联淀粉微球,并以二乙胺基乙基修饰了微球的表面电荷。同时研究了制备淀粉微球的制备工艺和改性工艺,成功制备了适合细胞培养用的淀粉微载体。利用扫描电镜(SEM)、红外(FT-IR)、X射线衍射仪(XRD)对微球进行了表征。结果表明,最佳制备条件为:淀粉溶液浓度为7%,搅拌速度为500 rpm,交联剂用量为8%,水油相体积比1∶6;微球表面修饰最佳条件为:加入微球质量2倍体积的3.5 mol/L NaOH溶液和2.5 mol/L DEAE-HCl溶液,60℃密闭反应4 h。对比Cytodex-1微载体,培养CHO-K1细胞至144 h时,淀粉微载体表面细胞密度达到1.8×10~6cells/mL,且两者培养效果相似,表明了自制淀粉微载体是一种潜在的贴壁细胞培养用高分子材料。更多还原

【Abstract】 The cross-linked starch microsphere(PCSM) was synthesized from potato starch by W/O emulsion method at room temperature,while its surface charge was modified with 2-dimethylaminoethyl.Meanwhile,the preparation and modification processes were studied,and the suitable starch microcarrier was successfully made for cell cultivation.The microspheres were characterized by scanning electron microscopy(SEM),Fourier translation infrared spectrum(FT-IR)and X-ray diffraction(XRD).The results showed that the optimal parameters for the preparation were as follows:the starch solution concentration of 7%,the mixing speed of 500 RPM,the crosslinking agent dosage of 8%,and the oil/water volume proportion of 1∶6.The optimal parameters for the modification process were found:2 times the mass of microspheres in volume of 3.5 mol/L NaOH solution and 2.5 mol/L DEAE-HCl solution added,and then stirred at 60 ℃ for4 h.Compared with Cytodex-1,after culturing CHO-K1 for 144 h,the cell density on the surface of starch microcarrier reached 1.8 x 106cells/mL.The culturing effect remained similar for both microcarrier,suggesting that the homemade starch microcarrier is a kind of potential high polymer material for adherent cell culture.更多还原