【摘要】 目的探讨大气颗粒物PM_2.5对人皮肤成纤维细胞（human skin fibroblast,HSF）分泌IL-6和TGF-β1的影响及胶原多肽（collagen polypeptide,CP）的干预作用。方法将HSF分为PM_2.5组和CP干预组。PM_2.5组用0、25、50、100、200μg/ml的PM_2.5与HSF培养48 h;CP干预组用100μg/ml PM_2.5和0、15、30、60μg/ml CP与HSF共培养48 h。采用ELISA方法测定细胞上清液中IL-6、TGF-β1含量。结果随着PM_2.5浓度升高（0、25、50、100、200μg/ml）,PM_2.5组细胞上清液中的IL-6含量也逐渐升高,TGF-β1含量则逐渐下降,两者都存在一定的剂量-效应关系,各炎性因子浓度差异有统计学意义（P<0.05）;在PM_2.5浓度为100μg/ml时,随着CP浓度升高（0、15、30、60μg/ml）,PM_2.5+CP组IL-6浓度逐渐下降,TGF-β1含量逐渐增加,两者都存在一定的剂量-效应关系,各炎性因子浓度差异有统计学意义（P<0.05）。结论 PM_2.5能促进皮肤炎症,可能与上调IL-6、下调TGF-β1有关;而CP能通过抑制上述作用,缓解PM_2.5对皮肤致炎作用。更多还原

【Abstract】 Objective To investigate the effect of PM_2.5 on secretion of interleukin 6（ IL-6） and transforming growth factor β1（ TGF-β1） by human skin fibroblasts（ HSF）,and the protective effect of collagen polypeptide（ CP） from insults of PM_2.5. Methods Human skin fibroblasts were co-cultured with PM_2.5 0,25,50,100,200 μg/ml( PM_2.5 groups) or PM_2.5 100 μg/ml plus 0,15,30,60 μg/ml CP( PM_2.5 + CP groups) for 48 h. The secretion of IL-6 and TGF-β1 were measured by ELISA method. Results With increasing concentration of PM_2.5 ,the secretion of IL-6 increased and the secretion of TGF-β1 decreased gradually in a dose-effect manner（ P < 0. 05）. Compared to PM_2.5 group,with increasing concentration of CP the secretion of IL-6 decreased and TGF-β1 increased gradually in PM_2.5 + CP groups in a dose-effect manner（ P < 0. 05）. Conclusion PM_2.5 can induce skin inflammation,which is associated with up-regulation of IL-6 and down-regulation of TGF-β1,and CP can inhibit the effect of PM_2.5 and alleviate the skin inflammation.更多还原