【摘要】 低丰度蛋白是生物体中重要的活性物质,参与新陈代谢、转录和翻译等多种生理及病理过程,因此对低丰度蛋白的研究具有重要意义。组合肽配体库技术(CPLL)是富集低丰度蛋白的一种有效方法,通过CPLL富集作用可以实现低丰度蛋白的检测与鉴定。该文总结了CPLL应用于不同领域中(包括人体、动物源、植物源等蛋白研究方面)低丰度蛋白样品的富集研究,可为CPLL的进一步应用提供参考。更多还原

【Abstract】 The low-abundance proteins play an important role in the biological activity of living organisms,involving in many physiological or pathological processes,such as metabolism,transcription and translation. Therefore,the analysis and identification of low-abundance proteins are vital in the study of proteomics. Combinatorial peptide ligand library( CPLL) is an effective method for the enrichment of low-abundance proteins,and the detection and identification of low-abundance proteins could be achieved after treated by CPLL. According to studies on the CPLL reported in recent years,its applications in different fields including the studies on low-abundance proteins in human,animal and plant protein samples were reviewed. The main purpose of this review is to provide a reference for future researches and applications of CPLL.更多还原