【摘要】 建立了高效液相色谱同时测定SO₂处理前后无核葡萄干中4种B族维生素(V_B1,V_B2,V_B3和V_B6)的分析方法。考察了流动相组成、p H值及梯度洗脱程序对分离效果的影响。最终采用pH 4.4缓冲溶液和乙腈作为流动相进行梯度洗脱,4种B族水溶性维生素的线性范围为0.1²0 mg/L,r2>0.999,检出限为0.013⁰.064 mg/L,方法的平均回收率为83.0%¹01.4%,相对标准偏差为0.6%⁴.8%。同时利用该法对SO₂处理前后无核葡萄干中4种维生素含量的影响进行研究。结果表明,空白无核葡萄干中V_B6的含量最大,V_B1含量较低;但SO₂处理对葡萄干中V_B1的含量影响最大,其它3种B族维生素的含量变化不大。该方法简便,快速,准确性和重现性均较好,可用于葡萄干中B族维生素的测定。更多还原

【Abstract】 A RP- HPLC method was developed for the determination of four B- vitamins( V_B1,V_B2,V_B3,V_B6) in currant after treated with SO2. The effects of mobile phase,p H value and gradient elution procedure on chromatographic separation were studied. The mobile phase was a mixture of p H4. 4 buffer solution and acetonitrile. Under the optimal conditions,the detection limits of four B- vitamins in currants were in the range of 0. 013- 0. 064 mg / L. The linear ranges were between 0. 1mg/L and 20 mg/L with correlation coefficients（r²） larger than 0. 999. The average recoveries for the method were in the range of 83. 0%- 101. 4% while RSDs were in the range of 0. 6%- 4. 8%.At the same time,the contents of these vitamins before and after treated with SO2 were investigated.The results showed that the content of V_B6 in currant was the highest and that of V_B1 was lower. But the change of V_B1 content in currant treated with SO₂ was the largest,and the contents of other three species of B- vitamins changed very little. The method showed the advantages of simplicity,rapidness,accuracy and reproducibility,and was suitable for the determination of B- vitamins in currants.更多还原