【摘要】 目的观察艾灸对实验性RA滑膜组织mPD-1表达的影响,深入探索阐释艾灸治疗RA抗炎效应的作用机理。方法健康成年SD大鼠45只,随机分为空白对照组、RA模型组、艾灸治疗组,每组各15只。采用弗氏完全佐剂进行RA模型复制。在造模后第7天,对艾灸组大鼠采用麦粒灸"肾俞"、"足三里"各5壮,每天1次,6天为1疗程,共治疗3个疗程。分别于造模前1天和造模后的第7天及治疗第21天测量各组大鼠的右后足容积,并计算其足肿胀度。并对其滑膜组织进行病理形态学检测,分级评分,用免疫组化法测定滑膜组织中mPD-1的表达。结果艾灸"肾俞"、"足三里"可明显改善RA模型大鼠的足肿胀度;艾灸能有效降低关节滑膜组织、纤维组织增生,炎性细胞浸润(P<0.01)及滑膜组织mPD-1表达(P<0.01)。结论艾灸对实验性RA具有明显的抗炎效应;艾灸通过调节滑膜组织mPD-1的表达,可能是其发挥抗炎效应的重要机制之一。更多还原

【Abstract】 Objective To observe the effect of moxibustion in the experimental RA rats and to explore the anti-inflammatory effect regulatory mechanism of mPD-1 for revealing the mechanism of moxibustion in the treatment of RA more depth.Methods A total of 45 SD rats(200±20 g weight) were randomized blank-control group(blank group),RA model group(model group) and moxibustion treatment group(moxibustion group) with 15 cases in each.RA model was established by injection of Freund’s Complete Adjuvant into the animal’s bilateral joint cavities in the first day of the experiment.In the 7th day after building,moxibustion was applied to "Shenshu"(BL23) and "Zusanli"(ST36),5 cones every time,once daily(except Sundays),3 weeks altogether.Measuring the right foot swelling in the experiment first day(before building) and on the 7th,28th day.Using the immunohistochemical method to detect the expression of mPD-1 in synovial tissue.Results Moxibustion on "Shenshu"(BL23) and "Zusanli"(ST36) acupoints could obviously improve RA rats’ right foot swelling.The result of showed that Moxibustion can reduce synovial inflammation and mPD-1 con ent significantly(P<0.01).Conclusion Moxibustion shows obvious anti-inflammatory effect in treating experimental RA.Moxibustion may adjust the expression of mPD-1 in synovial tissue to exerting the anti-inflammatory effects.更多还原