目的研究乙型肝炎病毒(Hepatitis B virus,HBV)感染与胆管癌(Cholangiocarcinoma,CC)的关系,并明确其与肝内胆管癌(Intrahepatic cholangiocarcinoma,ICC)和肝外胆管癌(Extrahepatic eholangiocarcinoma,ECC)发生的关系。方法采用免疫组化、巢式PCR对52例CC(21例肝内胆管癌和31例肝外胆管癌)石蜡包埋组织进行HBV相关蛋白及基因的检测。结果 21例ICC组织中HBsAg阳性表达率为23.8%(5/21),HBcAg阳性表达率为19.0%(4/21),HBV X基因检出率为33.3%(7/21)。在31例ECC组织中均未检出HBsAg、HBcAg及HBV X基因。结论 HBV感染与ICC发生关系密切,但可能非ECC发生的危险因素。HBV X基因的整合可能参与肝内胆管上皮细胞的癌变过程。
【英文摘要】
Objective To investigate the importance of hepatitis B virus(HBV)infection in the pathogenesis of cholangiocarcinoma(CC)and further clarify the correlation between the occurrence of intrahepatic and extrahepatic cholangiocarcinoma.Methods HBV protein and gene of 52 formalin fixed,paraffin embedded tissues with CC were detected by immunohistochemistry and nest PCR.Results Hepatitis B virus X gene was detectable in 33.3%(7/21)of 21 intrahepatic cholangiocarcinoma cases.Hepatitis B surface antigen(HBsAg)was de...
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