【摘要】 目的探讨细胞自噬对糖尿病神经病理性疼痛(DNP)大鼠脊髓内细胞凋亡的影响。方法 SD大鼠腹腔注射链脲霉素(STZ)建立DNP模型,随机分为DNP组、DNP+雷帕霉素组(DNP-R组)、DNP+3-甲基嘌呤组(DNP-M组)及对照组(C组)。DNP-R及DNP-M组造模后鞘内注射雷帕霉素或3-甲基嘌呤(3-MA)。采用Western-Blot测定脊髓内LC3-II、Bcl-2、Caspase-3蛋白表达,采用TUNEL检测脊髓内细胞凋亡率。结果与DNP组相比,DNP-R组LC3-II表达显著上调,Caspase-3及Bcl-2蛋白表达显著下调,凋亡细胞比例显著减少,DNP-M组LC3-II表达显著下调,Caspase-3及Bcl-2蛋白表达显著上调,凋亡细胞比例显著增加。结论 DNP大鼠脊髓内自噬激活抑制细胞凋亡,反之自噬抑制促进细胞凋亡,表明两者间可能存在相互影响。更多还原

【Abstract】 Objective To investigate the effect of autophagy regulation on apoptosis of spinal cord cell in diabetic neuropathic pain(DNP)rats. Methods The SD rats were intraperitoneally injected with Streptozotocin to establish DNP model,and were randomly divided into 4 groups:DNP group,DNP-Rapamycin(DNP-R)group,DNP-3-M(DNP-M)group and the control(C)group. Rapamycin or 3-MA was intrathecally injected in rats in DNP-R group and DNP-M group. The protein expression of LC3-II,Bcl-2 and Caspase-3 was detected by Western blot assay,the cell apoptosis was detected by TUNEL assay. Results Compared with DNP group,the expression of LC3-II was significantly up-regulated,while the expression of Caspase-3 and Bcl-2 were significantly down-regulated in DNP-R group. For DNP-M group,the expression of LC3-II was significantly down-regulated,while the expression of Caspase-3 and Bcl-2 were significantly up-regulated. The cell apoptosis was significantly reduced in DNP-R group,and significantly increased in DNP-M group. Conclusion There might be a crosstalk between autophagy and apoptosis in spinal cord DNP model. The activation of autophagy might inhibit the apoptosis,while the depression of autophagy might increase the apoptosis of spinal cord cells.更多还原