Objective To assess the effects of two different methods on differential cell count and thedetection on inflammatory mediators in the process of induced sputum. Methods Induced sputum samples fromoutpatients with respiratory diseases were collected. Each sample was equally divided into two parts. One portionwas processed at a regular way by incubation at 37 ℃ for 15 min in 0.1% dithiothreitol(DTT). Another wasprocessed at 0 ℃ for 60 min in 0.1% DTT. The cell pellet was used for the cytology analysis by HE s...
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