【摘要】 目的:研究CUEDC2在糖尿病肾病小鼠肾脏中的表达、定位及与肾功能相关性。方法:选用db/db小鼠为实验组,db/m小鼠为对照组,相同饲养条件下,24周时取组织样本;采用PAS,HE及Masson染色观察肾脏病理改变;Western Blot,免疫组织化学法,Real time PCR检测肾组织CUEDC2的表达、定位;由本院检验科测定血肌酐、24 h尿白蛋白。结果:CUEDC2蛋白表达定位于肾小管上皮细胞中。24周时,db/db小鼠出现肾小球硬化,肾间质增宽、炎症细胞浸润;较同期db/m小鼠,系膜基质明显扩张,肾小球及肾小管间质胶原染色增多,肾组织CUEDC2蛋白及mRNA均下调(P<0.05)。db/db小鼠肾脏中CUEDC2含量分别与24 h尿白蛋白与血肌酐呈明显负相关(P<0.05)。结论:CUEDC2在糖尿病小鼠肾脏中下调,并与尿白蛋白、血肌酐含量呈负相关。更多还原

【Abstract】 ObjectiveTo discuss the expression level of CUEDC2 protein and its connection with 24 h urinary albumin and serum creatinine in db/db mice with diabetic nephropathy.Methods db/db mice were selected as experimental groups(n=10),and db/m mice as control(n=10).All mice were fed in barrier facilities under the same conditions.At week 24,all were sacrificed and the samples were collected for analyses.The histological changes were assessed by Hematoxylin-Eosin(HE)staining,periodic acid-Schiff(PAS)staining and Masson′s trichrome(Masson)staining.The location and expression of CUEDC2 were measured by immunohistochemistry assays.24 h urinary albumin and serum creatinine were quantified by clinic lab in our hospital.Results Immunohistochemistry demonstrated that CUEDC2 was mainly located in the medulla tubules plasma cells.The results of HE staining revealed that there appeared glomerular number decreased,atrophy and inflammatory cell infiltration in the mice kidney of diabetic nephropathy group at the 24th week.The mesangial matrix expansion and renal tissue collagen deposition were significantly up-regulated in db/db mice compared with the normal control.As compared with the control group,the CUEDC2 protein expression and m RNA expression in db/db mice were significantly decreased than that in db/m mice(both P<0.05),and 24 h urinary albumin and serum creatinine were significantly increased.The correlation analysis showed CUEDC2 was negatively correlated with 24 h urinary albumin and serum creatinine(both P<0.05).ConclusionThe expression of CUEDC2 in diabetic nephropathy mice kidney is significantly decreased and negatively correlated with the levels of 24 h urinary albumin and serum creatinine更多还原