【摘要】 目的初步了解成都市流感病毒主要宿主感染状况,为流感防控提供科学依据。方法以2014年成都市疾病预防控制中心门诊体检人群和成都市区生猪屠宰场内生猪为调查对象,分别采集人血清50份和猪血清49份。以同期分离的A型H1N1、H3N2、B-Victoria和B-Yamagata代表毒株为抗原进行血凝抑制(HI)试验,检测血清中的相应抗体。结合当地流感流行情况,比较分析宿主中及宿主间各型病毒感染状况。结果流感样监测病例中流感病毒各亚型间的阳性检出率差异有统计学意义(χ~2=50.393,P<0.001),其中B-Yamagata阳性检出率最高(6.03%)。人血清中B-Yamagata和B-Victoria的HI抗体阳性率高于其它流感病毒(χ~2=239.104,P<0.001)。猪血清中A(H1N1)和B-Yamagata流感病毒抗体阳性率高于其它流感病毒(χ~2=231.642,P<0.001)。人血清中各型和各亚型的HI抗体滴度均高于猪血清中的滴度,且差异均有统计学意义(χ~2=17.099,P=0.008;χ~2=31.581,P<0.001;χ~2=57.623,P<0.001;χ~2=5.713,P=0.032)。结论2014年成都市疾病预防控制中心体检门诊体检人群中A(H1N1)和B型流感病毒的感染率较高,人群中较高的BYamagata HI抗体阳性率并未形成有效的免疫屏障阻止病原持续循环。猪可能是人B型流感病毒的潜在宿主。更多还原

【Abstract】 Objective To preliminarily investigate the infection status of influenza virus in main hosts in Chengdu City so as to provide a scientific basis for influenza prevention and control.Methods Fifty check-up persons from the Physical Examination Department of Chengdu Municipal Center for Disease Control and Prevention and 49 swines from the slaughterhouse of urban area in Chengdu in 2014 were investigated.Serum samples were collected and haemagglutination inhibition (HI) assay with representative strains (A (H1 N1),H3 N2,B-Victoria and B-Yamagata) isolated in the same period as antigens were carried out to detect the corresponding antibodies.Combined with the local epidemic situation of influenza,the infection status in and between the hosts were analyzed.Results The positive detection rates of subtypes of influenza viruses in the influenza-like cases were statistically significant (χ~2= 50.393,P<0.001),with the highest positive rate (6.03%) for influenza B-Yamagata lineage virus.As for human serum samples,HI antibody positive rates of influenza B-Yamagata and B-Victoria lineage virus strains were higher than those of the other influenza viruses (χ~2= 239.104,P<0.001),while the positive rates of influenza A (H1 N1) and B-Yamagata lineage virus strain in swine serum samples were higher than those of the other influenza viruses (χ~2= 231.642,P< 0.001).Generally,the antibody titers of A (H1 N1),H3 N2,B-Victoria and B-Yamagata were statistically higher in human serum samples than in swine serum samples (χ~2= 17.099,P = 0.008; χ~2= 31.581,P<0.001; χ~2= 57.623,P<0.001; χ~2= 5.713,P = 0.032).Conclusions The check-up persons from the Physical Examination Department of Chengdu Municipal Center for Disease Control and Prevention in 2014 get higher infection rates of influenza A (H1 N1) and B virus and the infection of B-Yamagata in crowd fails to form an effective immune barrier to prevent pathogen cycling.Swine may be a potential host of human influenza B virus.更多还原