【摘要】 目的用高效液相色谱法测定2型糖尿病患者血清中五种磷脂含量的变化。方法取70例2型糖尿病患者血清、81例正常对照组人群血清;采用正向高效液相色谱法测定血清中的磷脂酰丝氨酸(PS)、磷脂酰肌醇(PI)、磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、神经鞘磷脂(SM)的含量,并做对比研究。结果 2型糖尿病患者体内PS[(0.139±0.031)mg/L vs.(0.154±0.029)mg/L]、PI[(0.021±0.016)mg/L vs.(0.027±0.017)mg/L]、PC[(0.150±0.039)mg/L vs.(0.385±0.022)mg/L]、PE[(0.070±0.026)mg/L vs.(0.087±0.044)mg/L]含量均低于正常人,差异有统计学意义(均P<0.05),SM含量[(0.039±0.026)mg/L vs.(0.044±0.028)mg/L]与正常人比较差异无统计学意义(P>0.05)。结论 2型糖尿病患者血清中PS、PI、PC、PE含量呈现下降趋势,提示这四种指标对糖尿病患者在正常人群中的筛查起到了辅助性作用。更多还原

【Abstract】 Objective To determine the changes of serum contents of five phosphatides in patients with type 2 diabetes mellitus using high performance liquid chromatography(HPLC). Methods Serum samples were collected from 70 patients with type 2diabetes mellitus and 81 normal controls for determination of phosphatidylserine(PS),phosphatidylinostiol(PI),phosphatidylcholine(PC),phosphatidylethanolamine(PE) and sphingomyelin(SM) by HPLC,and for comparison between the two groups.Results The serum contents of PS((0. 139 ± 0. 031) mg/L vs.(0. 154 ± 0. 029) mg/L)),PI((0. 021 ± 0. 016) mg/L vs.(0. 027±0.017) mg/L),PC((0.150±0.039) mg/L vs.(0.385±0.022) mg/L) and PE((0.070±0.026) mg/L vs.(0.087±0. 044) mg/L) were lower in the patients with type 2 diabetes mellitus than in the normal controls,with statistically significant differences(all P<0.05). No statistically significant difference was discovered in the serum content of SM((0.039±0.026) mg/L vs.(0.044±0.028) mg/L) between the two groups(P> 0.05). Conclusions Serum PS,PI,PC and PE decline in the patients with type 2 diabetes mellitus,and they may play adjuvant roles in the screening of diabetes mellitus among normal population.更多还原