【摘要】 目的了解中国部分地区基于社区人群的未经抗病毒治疗的乙型肝炎(乙肝)病毒(HBV)表面抗原(HBsAg)阳性孕妇产前HBV血清学和病毒学特点。方法该研究从广西、江苏、河南地区入组1741例HBsAg阳性孕妇。所有孕妇的临产前血清样本采用Abbott Architect i2000和Abbott Architect m2000分别检测HBV血清学标志物及HBV DNA水平;采用型特异性引物巢式聚合酶链反应(n PCR)法进行HBV基因型分型。结果 1741例HBsAg阳性孕妇中,HBeAg阳性占37.0%(645/1741),HBeAg阴性孕妇占63.0%(1096/1741)。HBeAg阳性孕妇产前HBsAg滴度、HBV DNA水平以及B基因型孕妇所占比例均高于HBeAg阴性孕妇,但年龄低于HBeAg阴性孕妇。HBeAg阳性孕妇的HBsAg滴度和HBV DNA水平呈正相关(r=0.790,P<0.001),HBeAg滴度和HBV DNA水平也呈正相关性(r=0.564,P<0.001)。而HBeAg阴性孕妇的HBsAg滴度和HBV DNA水平无相关性(r=0.020,P=0.517)。结论未经抗病毒治疗的HBsAg阳性孕妇在不同HBeAg状态下,血清HBV DNA水平及HBsAg滴度分布各不相同。对于HBeAg阳性孕妇人群,HBeAg滴度、HBsAg滴度可能成为HBV DNA水平的替代指标。更多还原

【Abstract】 Objective To investigate the prenatal serological and virological characteristics of HBsAg-positive pregnant women without in some areas in China. Methods A total of 1741 HBsAg-positive pregnant women were recruited from Guangxi, Jiangsu and Henan provinces. Serum samples were collected before labor for evaluation of pre-delivery ALT, HBV serological markers and HBV DNA.HBsAg, HBeAg and HBV DNA levels were tested by Abbott Architect i2000 and m2000 systems, respectively. HBV genotyping was performed by a method based on nested polymerase chain reaction(n PCR) established in our lab. Results Among 1741 HBsAg positive women, 37.0%(645/1741) were HBeAg positive and 63.0%(1096/1741) were HBeAg negative, respectively. The median titers of HBsAg, HBV DNA levels and the proportion of genotype B in HBeAg-positive women were higher than those of HBeAg-negative women, but the age of HBeAg-positive women was younger than that of HBeAg-negative ones. The proportion of HBeAg-positive women was gradually declining with the increase of age. HBsAg titers and HBeAg titers were positively correlated with HBV DNA levels in HBeAg-positive group(r=0.790, P<0.001; r=0.564, P<0.001). However, there was no correlation between HBsAg titers and HBV DNA levels in HBeAg-negative group(r=0.020, P =0.517). Conclusion The serological and virological characteristics of treatmentna?ve HBsAg-positive women differed by the HBeAg status.Quantitative HBsAg titer and HBeAg titer might be surrogate markers for HBV DNA levels in HBeAg-positive women.更多还原