【摘要】 目的 探究大鼠原位肝癌纤维间质成分的特点及其与MR延迟强化的关系。方法 4只Wistar大鼠原位肝癌模型行肝脏MR扫描及多期延迟增强扫描,取12个肝癌标本行HE、苦味酸-天狼猩红染色、Verhoeff Van-Gieson染色、Gordon-Sweets染色及α-SMA免疫组化染色,观察肿瘤内及周边纤维间质特点及MR延迟强化特点。结果 肝细胞癌内胶原纤维分布于病灶内的纤维间隔中;弹力纤维分布于肿瘤内的纤维间隔及动脉血管壁;网状纤维分布于癌灶内、汇管区及假包膜纤维组织中。胶原、弹力、网状纤维的平均面密度值分别为0.102±0.020、0.063±0.018、0.109±0.032,胶原纤维及网状纤维与弹力纤维间有统计学差异(P<0.01),胶原纤维与MR延迟强化间正相关(P<0.05)。结论 大鼠肝癌MR延迟强化与病灶内胶原纤维关系密切,MR可对肝癌内胶原纤维进行无创评估。更多还原

【Abstract】 Objective To explore the fibrotic stroma characteristics of orthotopic liver cancer in rat and the relationship with MR delayed contrast enhancement.Methods 4 Wistar rats with orthotopic liver cancer underwent conventional triphasal and longer-delayed contrast-enhancement MR scanning.12 HCC specimens obtained from the four Wistar rats were sliced and stained with HE,picric-sirius red,Verhoeff Van-Gieson elastic fiber,Gordon-Sweets reticular fiber and anti-α-SMA immunohistochemical staining.The relationshp between intratumoral fibrotic stroma and MR delayed phases of the 12 tumors were analyzed.Results Collagen fiber was expressed mainly in tumor fibrous septum.Elastic fiber distributed in tumor fibrous septum and the artery wall.Reticular fiber distributed in fibrous septum of the regenerative nodules,portal area and tumor psuedocapsule.Integrated optical density(IOD) value of collagen fiber,elastic fiber and reticular fiber analyzed by Image-pro Plus6.0 were 0.102±0.020,0.063±0.018 and 0.109±0.032,respectively.As a result,the amount of collagen and elastic fibers were statistically different(P<0.01),and so were reticular fibers and elastic fibers(P<0.01).And there was a positive correlation between collagen fiber and MR delayed contrast enhancement(P<0.05).Conclusion MR delayed contrast enhancement of hepatic cancer in rat is closely associated with the collagen fiber,thus this fiber in liver cancer can be evaluated noninvasively by MRI.更多还原