Objective: To construct the expression plasmid of human Sestrin 3( h Sesn3) gene and identify expression and localization of the fusion protein in osteosarcoma MG-63 cells. Methods: Total mRNA was extracted from Hela cells,and the c DNA was reversely transcripted. The h Sesn3 coding sequence was amplified by polymerase chain reaction( PCR) and sub-cloned into p EGFP-C1 empty vector. After the PCR fragment was identified by double restriction enzymes digestion and sequenced,the plasmid was transiently transf...
【基金】
国家自然科学基金资助项目(编号:31201053,81372337)
【更新日期】
2017-08-25
【分类号】
R738.1
【正文快照】
Modern Oncology 2017,25(17):2699-2702【修回日期】2017-03-27Sestrins(Sesns)是一类高度保守的应激蛋白家族,当细胞暴露在不同的环境压力情况下(氧化应激和DNA损伤)Sesns起到保护细胞的作用。其中Sesns主要通过参与调控AMPK和m TOR信号通路维持细胞稳态。近来的研究发现,Ses
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