【摘要】 目的:探讨明胶酶A(MMP-2)在乳腺正常组织、不典型增生、原位癌、浸润性癌组织中的表达水平,分析其与浸润性乳腺癌临床相关病理指标及术后生存情况的相关性。方法:收集正常乳腺组织20例、不典型增生28例、导管内癌40例、浸润性乳腺癌标本80例,用免疫组化SP法检测各组MMP-2的表达,分析其在浸润性乳腺癌中的表达水平与肿瘤大小、腋窝淋巴结受累情况、TNM分期、组织学分级、ER、PR、HER-2等临床病理指标之间的相关性;并分析其对乳腺癌患者术后生存状况的影响。结果:MMP-2在乳腺正常组织(阳性率20.0%)、乳腺不典型增生组织(阳性率21.4%)、原位癌组织(阳性率46.3%)、浸润性乳腺癌组织(阳性率71.3%)的表达依次增高;MMP-2阳性率在乳腺原位癌与不典型增生、浸润性癌与原位癌之间具有统计学差异(P<0.05)。在浸润性乳腺癌中,不同临床分期及淋巴结转移亚组MMP-2阳性率比较有统计学差异(P<0.05)。在原位癌中,MMP-2染色阳性组5年无病生存率(47.4%)低于阴性组(95.2%);在浸润性乳腺癌中,MMP-2染色阳性组5年无病生存率(56.9%)低于阴性组(86.5%),均有统计学差异(P<0.05)。结论:MMP-2参与了乳腺癌发生、发展的过程,并与乳腺癌预后相关,可作为预测乳腺癌预后的潜在肿瘤标志物。更多还原

【Abstract】 Objective:To study the expression of matrix metalloproteinase-2(MMP-2)in normal tissue of breast,atypical hyperplasia,ductal carcinoma in situ and invasive carcinoma,and analyze the associations with pathological features and clinical outcome in human breast cancer.Methods:Expressions of MMP-2 in 20 cases of normal breast tissue,28 cases of atypical hyperplasia,40 cases of ductal carcinoma in situ(DCIS)and 80 cases of invasive carcinoma were determined by IHC(immune ohistochemistry)technique respectively.The level of MMP-2 expression in 80 cases of invasive breast cancer was analyzed against the clinical and pathology features such as tumor diameter,lymph node status and so on.All the 120 cases of breast carcinoma were followed–up.Results:The expression of MMP-2 increased from normal tissue of breast(the positive rate 20.0%)to atypical hyperplasia(the positive rate21.4%)and DCIS(the positive rate 46.3%)and invasive breast cancer(the positive rate 71.3%).There was statistical difference between DCIS and atypical hyperplasia(P<0.05).While there was statistical difference between invasive carcinoma and DCIS(P <0.05).The expression of MMP-2 had statistically significant differences in some groups of different clinical stage and lymph node metastasis(P<0.05).Disease free survival rate of ductal carcinoma in situ group with MMP-2 positive expression(47.4%)was lower than the negative group(95.2%)(P<0.05).Disease free survival rate of the invasive carcinoma group with MMP-2 positive expression(56.9%)was lower than the negative group(86.5%)(P<0.05).Conclusions:MMP-2 participate the course of breast cancer.It may be a prognostic indicator of breast cancer.更多还原