【摘要】 目的探讨血清miRNA-720和miRNA-484是否能作为非侵入性的结肠癌生物标记。方法采用实时荧光定量PCR检测104例结肠癌患者(分为Ⅰ~Ⅱ期组和Ⅲ~Ⅳ期组)以及60例健康对照血清中miRNA-720和miRNA-484的表达。通过ROC曲线分析两种miRNA对不同分期结肠癌患者的诊断价值。结果与健康对照组相比,miRNA-720在Ⅰ~Ⅱ期组上调接近2倍(t=1.997,P<0.05),在Ⅲ~Ⅳ期组上调3倍左右(t=2.133,P<0.05);miRNA-484在Ⅰ~Ⅱ期组下调约2倍(t=2.585,P<0.05),在Ⅲ~Ⅳ期组上调超过3倍(t=3.416,P<0.01)。两种miRNA用于诊断结肠癌的ROC曲线下面积(AUC)分别为0.76(95%CI;0.67~0.86)和0.79(95%CI:0.69~0.89);两者联合使用可增至0.87(95%CI:0.77~0.96)。结论血清miRN A-720和miRNA-484可作为非侵入性生物标记对结肠癌做出早期诊断,并能区别出不同分期的结肠癌。更多还原

【Abstract】 Objective To investigate whether serum miRNA-720 and miRNA-484 can be used as non-invasive biomarkers of colon cancer.Methods Real time-polymerase chain reaction was used to detect the expressions of miRNA-720 and miRNA-484 in serum from 104 colon cancer patients(divided into Ⅰ~Ⅱ-stage group and Ⅲ~Ⅳ-stage group) compared with 60 additional healthy controls.ROC curve was performed to analyse the diagnostic usefulness of both miRNAs distinguishing between different stages of colon cancer patients.Results Compared with healthy controls,the.miRNA-720 was upregulated close to 2 times in Ⅰ~Ⅱ-stage group(t=1.997,P<0.05),and was upregulated about 3 times in Ⅲ~Ⅳ-stage group(t=2.133,P<0.05).The miRNA-484 was downregulated almost 2 times in Ⅰ~Ⅱ-stage group(t=2.585,P<0.05),but was upregulated over 3 times in Ⅲ~Ⅳ-stage group(t= 3.416,P<0.01).The area under the ROC curve(AUC) for the diagnosis of colon cancer were 0.76(95%GI:0.67~0.86) and 0.79(95%CI:0.69~0.89) respectively.The combined use of both miRNAs could make the AUC up to 0.87(95%CI:0.77~0.96).Conclusion Serum miRNA-720 and miRNA-484 can be used as non-invasive biomarkers to diagnose early colon cancer.They can also distinguish between different stages of colon cancer patients.更多还原