【摘要】 流式细胞术是一门具有广泛科研以及临床应用价值的技术。在研究生教学中开设针对性的流式细胞术实验课,对研究生加深该技术的理解和掌握并有效用于课题研究具有重要意义。为此中国医科大学细胞生物学教研室在研究生细胞生物学实验课教学中设置了流式细胞术检测5-FU对大肠癌细胞系HCT116的细胞周期抑制作用和流式细胞术检测CD44在大肠癌细胞系HCT116中的表达情况两个教学范例。研究生通过亲自参与细胞的收集,荧光的标记,样本的上机操作以及数据的分析等,不仅提高了研究生的动手操作能力,还培养了研究生的科研分析能力,为研究生在未来的科研和医疗工作中利用该技术奠定了基础。更多还原

【Abstract】 Flow cytometry is a technology with extensive scientific research and clinical application value. It is very important for graduate students to deepen the understanding and mastery of the technology and use it effectively in research. Two teaching examples were set on "cell cycle inhibition of 5-FU on colorectal cancer cell line HCT116 by flow cytometry"and "flow cytometry detection of CD44 expression in colorectal cancer cell line HCT116"in teaching postgraduate students biological experiment. The experiments not only improved the hands-on ability of the postgraduate students,but also cultivated their scientific research and analyzing ability by participating in the collection of cells,the labeling of the fluorescence,the operation of the samples and the analysis of the data. This course laid a foundation for postgraduate students to use the technology in future research and medical work.更多还原