【摘要】 试验以原代睾丸支持细胞为对象,通过胶原酶和胰酶的消化分离支持细胞,用HE染色及GATA-4免疫荧光对分离的支持细胞进行鉴定;利用q RT-PCR法检测不同浓度(0,0.1,1,10 mg/L)Na F对支持细胞骨架ARP2,ARPC3和ARPC4处理24 h后mRNA相对表达量,旨在研究氟对雄性生殖功能的影响。结果显示,ARP2在0.1 mg/L Na F组mRNA相对表达量显著降低(P<0.05),在10 mg/L Na F组mRNA相对表达量极显著降低(P<0.01);ARPC4在10 mg/L Na F组mRNA相对表达量显著降低(P<0.05);ARPC3的mRNA表达量没有显著变化。表明氟化钠通过引起支持细胞骨架相关基因相对表达量的下降,影响睾丸支持细胞的结构与功能,进而损伤睾丸的生精功能。更多还原

【Abstract】 To explore the effect of fluoride on male reproductive, primary sertoli cells were isolated by means of the enzyme digestion method with trypsin and collagenase. The survival rate and the purity of cells were detected with the HE and the immunohistochemical analysis of GATA-4. Then the cells were exposed to increasing Na F concentrations(0, 0.1, 1, 10 mg/L)for 24 h.The expression level and distribution of cytoskeletal gene ARP2, ARPC3 and ARPC4 were detected by quantitative real-time-polymerase chain reaction(q RT-PCR). The results showed that the relative expression of ARP2 mRNA in 0.1 mg/L Na F group decreased significantly(P<0.05), and the relative expression of mRNA in 10 mg/L Na F group significantly decreased(P<0.01), the relative expression of ARPC4 mRNA in 10 mg/L Na F group decreased significantly(P<0.05). The expression of ARPC3 mRNA did not change significantly. In conclusion, sodium fluoride can decrease the relative expression of cytoskeleton-related genes, affect the structure and function of testicular support cells, and then damage the testicular spermatogenic function.更多还原