Objective To develop a ultraviolet(UV)-visible spectrophotometry for rapid and accurate determination of bacterial biomass of serogroup W135 Neissria meningitidis. Methods The fermentation liquid of serogroup W135 N. meningitides was diluted serially, and determined for A value at optimal absorption wavelength. Meanwhile, the bacterial counts and wet/dry weights in the fermentation liquids at various concentrations were determined to plot a relation curve of A values against the wet/dry weights and bacteria...
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