Objective To analyze the sequence and polymorphism of exons 2 and 3 of BF gene in chicken. Methods DNA was isolated from the peripheral blood leukocytes(PBLs) of six Xiayan chickens and used as a template for amplification of exon 2, intron 2 and exon 3 of BF gene by PCR. The PCR products were cloned into vector pGM-T, and the constructed recombinant plasmid pGM-T-BF was identified by PCR, restriction analysis and sequencing. Bioinformatics software DNAStar was used to analyze multiple alignment of nucleoti...
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