目的比较白喉无毒突变体(cross-reactive material 197,CRM197)、白喉类毒素(diphtheria toxoid,DT)及破伤风类毒素(tetanus toxoid,TT)3种不同载体蛋白C群脑膜炎球菌荚膜多糖(group C meningococcal polysaccharide,GCMP)结合疫苗在小鼠体内的免疫原性。方法在碳二亚胺[N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride,EDAC]的缩合作用下,将TT和DT用1,6-己二酰肼(1,6-adipic acid dihydrazide,ADH)进行衍生,制备TTAH及DT-AH衍生物,GCMP再分别与TT-AH、DT-AH及CRM197共价结合制备结合物,经Sepharose 4FF凝胶柱分离纯化后,进行化学检测、相对分子质量测定及鉴别试验。用GCMP及3种结合物经皮下注射NIH小鼠,第0、14、28天各免疫1次,分别于首免后第7、21和35天经小鼠颈动脉采血,分离血清,ELISA法检测抗体效价。结果GCMP-TT和GCMP-DT的糖/蛋白比及多...
【英文摘要】
Objective To compare the immunogenicity of group C meningococcal glycoconjugate vaccines using cross-reactive material 197(CRM197), diphtheria toxoid(DT) and tetanus toxoid(TT) as protein carriers in mice. Methods TT and DT were derived with 1, 6-adipic acid dihydrazide(ADH)to prepare derivatives TT-AH and DT-AH. TT-AH, DTAH and CRM197 were covalently bound to GCMP in mediation of N-(3-dimethylaminoprophyl)-N′-ethylcarbodiimide hydrochloride(EDAC), and the prepared GCMP-TT, GCMP-DT and GCMP-CRM197 conjugate...
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