Phospholipase A1( plaA) is an enzyme that hydrolyzes phospholipids. We used bioinformatics methods to analyze plaA and its accessory protein gene( plaS),which will provide a basis for further study of phospholipase. We cloned and sequenced the plaA and plaS of Serratia marcescens,and the characteristics of nucleotide and amino acid sequence were analysed. Furthermore,we also predicted the secondary structure and tertiary structure of Pla A and Pla S protein. Phylogenetic trees were reconstructed using maxim...