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铅暴露对大弹涂鱼(Boleophthalmus pectinirostris)组织形态的影响
Effects of Pb2+ on tissue structure of liver,intestines and spleen in mudskipper Boleophthalmus pectinirostris
【摘要】 为了研究环境中铅离子(Pb2+)对大弹涂鱼肝、肠和脾组织结构的损伤,实验挑选体质量(21.80±2.70)g的健康大弹涂鱼96尾,分为4组,高剂量Pb2+处理组(2 mg/L)、中剂量Pb2+处理组(1 mg/L)、低剂量Pb2+处理组(0.5 mg/L)和空白组,进行毒性试验,分别于第12 h、48 h和96 h取样,对各组织器官进行组织切片分析。结果表明:随着Pb2+浓度的升高及处理时间的延长,肝组织出现组织水肿,部分细胞质空泡化、细胞核固缩;肠组织上皮细胞受损,杯状细胞明显增多;脾中淋巴细胞增多。研究表明,Pb2+暴露对大弹涂鱼肝、肠及脾组织结构均会造成伤害。
【Abstract】 In order to study the effects of Pb2+ on tissue structure of liver,intestines and spleen of fish,Mudskippers( 21. 80 ± 2. 70)g were randomly stocked into 16 tanks with 8 fish each in triplicate. The fish were exposed to 0,0. 5,1 and 2 mg/L Pb2+,and sampled on the 12th,48th and 96 thh. The results showed that Pb2+ exposure results in tissue edema,cytoplasm vacuolization and in liver,epithelial cell damage,gobiet cells increased in intestinal,and lymphocytes increased in spleen. The results indicated that Pb2+exposure can cause damage to the structure of liver,intestines and spleen of mudskipper.
【Key words】 Pb2+; Boleophthalmus pectinirostris; toxic mechanism; bio-concentration; structure;
- 【文献出处】 生物学杂志 ,Journal of Biology , 编辑部邮箱 ,2017年04期
- 【分类号】S917.4;X171.5
- 【被引频次】2
- 【下载频次】140