【摘要】 目的:利用丙型肝炎病毒(HCV)复制子细胞模型,体外研究重组人λ2干扰素(hIFNλ2)对HCV复制子的抑制作用。方法:构建重组质粒pcDNA3.1-hIFNλ2-HA,转染293T细胞,表达带有HA标签的hIFNλ2,表达后的上清通过Western印迹检测,然后稀释至不同浓度后作用于带有HCV复制子的Huh7.5细胞系,每隔24h取样并更换培养液,通过报告基因检测hIFNλ2对HCV复制子的抑制作用。结果:构建了pcDNA3.1-hIFNλ2-HA真核表达重组体,并在293T细胞中表达出hIFNλ2,该蛋白能有效抑制HCV复制子的复制。结论:hIFNλ2对HCV复制子具有抑制作用。更多还原

【Abstract】 Objective: To study the inhibitory effect of recombinant human interferon λ2(hIFNλ2) on HCV replicon in vitro by using cell culture system of HCV replicon. Methods: This study constructed recombinant plasmid pcDNA3.1-hIFNλ2-HA, and expressed the hIFNλ2 with HA tag in 293 T cells, then detected the supernatant of the cell by Western blot, and then used supernatant diluted with different ratio to effect on Huh7.5 cell culture system with HCV replicon, samples were collected every 24 hours and replaced the medium, finally using the report gene to detect the inhibitory effect of the hIFNλ2 in HCV virus replicon. Results: The eukaryotic expression recombinant plasmid pcDNA3.1-hIFNλ2 was successfully contructed, and hIFNλ2 was expressed in 293 T cells,and confirmed that the hIFNλ2 could inhibit the replication of HCV. Conclusion: The results proved that hIFNλ2 has a direct inhibitory effect on HCV replicon.更多还原