【摘要】 莱茵衣藻(Chlamydomonas reinhardtii)是一种三套基因组都可以进行遗传转化的模式生物,具有培养条件简单、生长速度快、光合效率高等优点,细胞核转化体系相对更为成熟,将其开发为生物反应器具有广阔的应用前景。该文对衣藻核基因组特点及转化机理、转基因衣藻的筛选方法、外源基因的表达以及影响因素等方面进行了综述。更多还原

【Abstract】 Chlamydomonas reinhardtii is a model organism whose three genomes can be genetic transformation.Chlamydomonas reinhardtii has many advantages such as simple culture conditions,fast growth and high photosynthetic efficiency.Currently,the nuclear transformation system of Chlamydomonas reinhardtii is a relatively mature technology,which displays wide application prospect for developing Chlamydomonas reinhardtii as bioreactor.In this review,nuclear genome characteristics and transformation mechanism of Chlamydomonas reinhardtii,screening method for transgenic Chlamydomonas reinhardtii,expression of foreign genes and influencing factors were summarized.更多还原