【摘要】 TAp73是P53家族的一员,能够调节肿瘤的生成、侵袭和转移。但是,TAp73调节肿瘤血管生成的作用备受争议。本研究将外源TAp73转染至P53基因表达状态不同的两株肺腺癌细胞系H1299(P53-null)和A549(wt P53)中,观察TAp73对肿瘤血管生成的作用并探讨与P53基因的关系。首先,使用RT-PCR和Western印迹验证转染效率。细胞划痕实验表明,TAp73在A549细胞中促进细胞迁移,而在H1299细胞中抑制细胞迁移。体外HUVEC血管形成结果表明,TAp73在A549细胞中促进细胞血管形成,而在H1299细胞中抑制细胞血管形成。同时,血管生成抑制蛋白1(VASH1)的表达水平,也分别升高或降低。本文研究结果表明,TAp73对肺腺癌细胞血管生成的作用依赖于P53基因的状态:在野生型P53基因存在时,TAp73促进血管生成,而在缺失P53基因的情况下,TAp73抑制血管生成。本研究对于TAp73作为肿瘤的潜在治疗靶点具有重要意义。更多还原

【Abstract】 TAp73 is a member of the P53 family,which can regulate the formation,invasion and metastasis of tumor.However,the role of TAp73 in regulating tumor angiogenesis is controversial.In this study,we transfected TAp73 into lung adenocarcinoma cell lines H1299(P53-null) and A549(wt P53)with different P53 gene status to observe the effect of TAp73 on angiogenesis and to explore therelationship with P53 gene.For this purpose,we first verified the transfection efficiency using RT-PCR and Western blotting.Wound healing assay showed that TAp73 promoted cell migration in A549 cell line and inhibited cell migration in H1299 cell line after TAp73 was overexpressed.Similarly,in vitro HUVEC tube formation assay showed that TAp73 promoted the ability of tube formation in A549 cell line and inhibited the ability of tube formation in H1299 cell line after TAp73 was overexpressed.At the same time,the expression level of VASH1 also increased in A549 cells or decreased in H1299 cells,respectively.Our results suggest that the effect of TAp73 on angiogenesis in lung adenocarcinoma cells depends on the status of P53 gene.TAp73 promotes angiogenesis in the presence of wild-type P53 gene,and TAp73 inhibits angiogenesis in the absence of P53 gene,which is perhaps significant for TAp73 as a potential therapeutic target in tumors.更多还原