【摘要】 目的:探讨肝细胞生长因子(HGF)和基质金属蛋白酶-9(MMP-9)在儿童过敏性紫癜(HSP)中表达的意义,探寻肾脏损害预测的敏感指标。方法:选取HSP与过敏性紫癜肾炎(HSPN)患儿各30例,并选取同期体检的健康儿童30名作为对照组,采用酶联免疫吸附试验法(ELISA)检测3组血清和尿液中的HGF及血清MMP-9含量,并采用比浊法检测24 h尿蛋白,分析HGF、MMP-9与24 h尿蛋白的相关性。结果:HSP组、HSPN组血清、尿液HGF,血清MMP-9及尿蛋白定量均高于对照组(P<0.05);HSPN组中Ⅱ~Ⅳ期患者血清HGF及Ⅰ~Ⅳ期尿液HGF、Ⅲ~Ⅳ期MMP-9及Ⅰ~Ⅳ期尿蛋白定量均高于HSP组(P<0.05);血清及尿液HGF与血清MMP-9无明显相关性(r=0.014,0.027,P>0.05);血清HGF与尿蛋白定量无明显相关性(r=0.032,P>0.05);尿液HGF、血清MMP-9与尿蛋白定量均呈正相关(r=0.412、0.302,P<0.05)。结论:尿液HGF、MMP-9均参与HSP、HSPN病情的发展,尿液HGF、MMP-9水平的监测有助于评估HSPN的病情及预后,但HGF与MMP-9之间无明显相关性。更多还原

【Abstract】 Objective: To investigate expressions of hepatocyte growth factor(HGF) and matrix metalloproteinases-9(MMP-9) in henoch-schonlein purpura(HSP), and to explore the sensitive indicators of nephritis. Methods: 30 children with henoch schonlein purpura nephritis(HSPN) were selected as the(HSPN group, and another 30 healthy children were selected as the control group. The enzyme-linked immunosorbent assay(ELISA) was applied to detect the HGF levels in serum and urine and serum contents of MMP-9,and turbidimetric method was used to detect the 24 h urine protein. Analyze the correlation of MMP-9 and HGF with 24 h urine protein.Results: The levels of urine and serum HGF, serum MMP-9 and urinary protein were higher in HSP and HSPN group than in control group(P<0.05). The levels of serum HGF of Ⅱ ~ Ⅳ stage patients, the urine levels of HGF of Ⅰ~ Ⅳ stage patients, the serum levels of MMP-9 of Ⅲ~ Ⅳ stage patients and the urinary protein levels of Ⅰ~ Ⅳ stage patients were higher in HSPN group than in HSP group(P<0.05). Serum and urine HGF levels had no significant correlation with MMP-9 serum levels(r=0.014, 0.027, P>0.05). Serum HGF had no significant correlation with urine protein quantitative(r=0.032, P>0.032). HGF urine level and MMP-9 serum level both had a positive correlation with urine protein quantitative(r=0.412, 0.302, P<0.05). Conclusion: The urine HGF and MMP-9 participates in the development of HSP and HSPN. The monitoring on the levels of urine HGF and MMP-9 may help to assess the state and prognosis of HSPN. But there was no significant correlation between HGF and MMP-9.更多还原