【摘要】 目的:探讨氢饱和生理盐水对大鼠脑缺血再灌注损伤模型的治疗效果及可能的作用机制。方法:SD大鼠(250-300g)随机分为3组(n=20):假手术组(Sham组),缺血再灌注损伤模型组(I/R组),氢饱和盐水治疗组(HS组)。采用大鼠线栓法右侧中动脉栓塞模型(MCAO模型),于模型90 min时拔出线栓进行再灌注,再灌注同时,I/R组腹腔给予生理盐水10 m L/kg,HS组腹腔给予氢饱和生理盐水10 m L/kg。24 h后,对各组大鼠进行神经功能缺陷评分。断头取脑后,TTC染色法检测脑组织梗死体积(n=10)。选取缺血半暗带处大脑皮层组织进行相关指标测定(n=10)。HE染色观察大鼠缺血半暗带脑组织形态结构,测定缺血半暗带区域氧化应激反应,选取指标为SOD,MDA;并观察缺血半暗带区域炎症反应,利用Elisa方法测定该处TNF-α,IL-6含量。结果:TTC染色证实,右侧MCAO明显诱导大鼠脑局灶性缺血(P<0.05)。与I/R组相比,HS组明显降低脑梗死体积(P<0.05),显著降低大鼠神经功能缺陷评分(P<0.05)。与I/R组相比,氢饱和生理盐水治疗后,很好的保持了缺血半暗带区域细胞结构完整性,明显提高了SOD含量(P<0.05),有效降低了MDA水平(P<0.05),并且减轻了炎症因子TNF-α,IL-6含量(P<0.05)。结论:氢饱和生理盐水可有效的治疗脑缺血再灌注损伤,其机制可能涉及氢气在缺血半暗带区域的选择性的抗氧化应激作用,以及抗炎症反应。更多还原

【Abstract】 Objective: The aim of this research was to study the effects on hydrogen-rich saline against the rat model of cerebral ischemia-reperfusion injury and elucidate the possible mechanisms. Methods: Sprague-Dawley rats weighing 250-300 g were distributed randomly into 3 groups(n=20): sham group,cerebral ischemia-reperfusion injury group(I/R group),and hydrogen-rich saline treated group(HS group). The model was induced by right middle cerebral artery occlusion(MCAO) via suture. Reperfusion was accomplished by withdrawing the suture after 90 min of ischemia. During the process of reperfusion,rats in I/R group were injected saline(10 m L/kg)intraperitoneally,and rats in HS group were injected hydrogen-rich saline(10 m L/kg) intraperitoneally. Neurological deficit scores were evaluated at 24 h after reperfusion. Then,the rats were decapitated,TTC tests were performed to detect the infarct volume of cortex(n=10). Ischemic penumbra was collected to analyze the involved indexes(n=10). We used HE staining to observe the morphology of cortical ischemic penumbra,and used SOD and MDA to evaluate the oxidative response in the region of cortical ischemic penumbra,and used TNF-α and IL-6 to evaluate the inflammatory response in cortical ischemic penumbra via Elisa test. Results: TTC staining test suggested that the model of right MCAO could induce focal ischemia of brain obviously(P<0.05). Compared with I/R rats,rats in HS group had decreased infarct volume of cortex(P<0.05) and lower neurological deficit scores(P<0.05). Rats in HS group maintained cellular structural integrity superiorly and had higher concentration of SOD(P<0.05),and lower concentration of MDA(P<0.05),TNF-α and IL-6(P<0.05) when compared to rats in I/R group. Conclusions: Hydrogen-rich saline could treat cerebral ischemia-reperfusion injury effectively,and the possible mechanisms involved the selectively antioxidant stress damage and effects of anti-inflammation in the region of cortical ischemic penumbra.更多还原