应用流式细胞检测技术测定了2014年春季南海北部海域浮游细菌和病毒丰度,研究了其水平和垂直分布特征并对其与环境因子的相关性进行了分析。结果表明,调查海区浮游细菌和病毒丰度分别介于1.28×10~4—9.96×10~5个/m L和4.69×10~5—5.39×10~7个/m L之间,二者丰度随水深的增加基本呈现逐渐下降的趋势,而水平分布趋势不明显。浮游细菌和病毒丰度与温度、p H和溶解氧显著正相关,与水深、盐度、活性磷酸盐、硅酸盐、硝酸盐和总氮则呈显著负相关关系(P<0.01),说明该海域细菌和病毒数量受到上述环境因子的共同调控。分析浮游细菌和病毒的相互关系发现,VBR(Virus to bacteria ratio)平均32.23,最小值位于S11站位25m层,最大值则位于S7站位75m层,分别为4.80和264.63,VBR值小于100的站位占到调查站位总数的95.6%。VBR值除与细菌呈显著负相关关系外(P<0.01),与其它环境因子相关性不明显(P>0.05),说明该海区细菌是病毒的主要寄主,病毒可能主要是以噬菌体的状态存在。
【英文摘要】
According to the concept of Microbial Loop,bacteria and virus play important role in organic matter recycling and energy flowing in marine ecosystem,and meanwhile influence many biogeochemical and ecological processes. Marine bacterial and viral ecology has become hotspot in current studies. The South China Sea( SCS) is one of the largest marginal seas in Northwest Pacific,and has already been proved to be oligotrophic. The SCS has attracted great attention due to its economic and strategic importance in re...