【摘要】 目的:为探讨壳聚糖对病原菌的抑制作用及对拮抗菌的影响,以期为拮抗菌Bacillus amyloliquefaciens BA-16-8应用于苹果采后的防腐保鲜提供理论基础。方法:以日本红富士为实验材料,采用体外及体内实验方法,研究不同浓度的壳聚糖对扩展青霉(Penicillium expansum)的抑制作用及解淀粉芽孢杆菌BA-16-8对扩展青霉产毒的影响。结果:浓度2%的壳聚糖溶液抗P.expansum和青霉病的能力最强,且不会影响拮抗菌B.amyloliquefaciens BA-16-8的生长。拮抗菌B.amyloliquefaciens BA-16-8的菌悬液及无细胞发酵液均可抑制P.expansum,且菌悬液的处理效果要优于无细胞发酵液。抑制产毒实验结果表明,B.amyloliquefaciens BA-16-8菌悬液及其无细胞发酵液均可显著抑制P.expansum发酵液中展青霉素的积累,但对单纯的展青霉素并无降解作用。结论:B.amyloliquefaciens BA-16-8同壳聚糖联合作用可抑制扩展青霉的活性及产毒能力。更多还原

【Abstract】 Objective: In order to discuss the inhibitory effect of chitosan on both pathogenic bacteria and biocontrol angent, and to provide theoretical basis for application of Bacillus amyloliquefaciens BA-16-8 in the antisepsis and fresh-keeping for postharvest apple. Method: Taking Fuji apple as experimental meterial, the inhibition effect on Penicillum expansum under different concentration of chitosan and patulin(PAT) removal ability by different concentration of fermentation broth of B.amyloliquefaciens BA-16-8 was determined in vivo and vitro. Result: As was shown in the result, the ideal concentration of chitosan was 2%, which could not only exbihit strong anti-fungal ability against P. expansum but also don’t inhibit growth of B. amyloliquefaciens BA-16-8. Moreover, both the fermentation broth and cellfree fermentation broth of B. amyloliquefaciens BA-16-8 could inhibit the growth of P.expansum, whereas the fermentation broth showed the stronger anti-fungal ability. Antifungal and patulin(PAT) removal experiments revealed that the cell-containing and cell-free fermentation broths of B.amyloliquefaciens BA-16-8 could significantly inhibit the PAT accumulation in fermentation broth of P. expansum. However, B. amyloliquefaciens BA-16-8 couldn’t degrade the patulin. Conclusion: Both the mycelia ability and patulinproducing ability of P. expansum would be significantly inhibited by combining the concentration of 2%(w/v) chitosan with fermentation broth of B. amyloliquefaciens BA-16-8.更多还原