【摘要】 目的探讨VEGF抗体对烫伤大鼠休克的影响。方法随机选取88只大鼠中的8只设定为治疗前组,余80只按照随机数表法随机分为治疗组(40只)与对照组(40只),并将治疗组与对照组大鼠建立为Ⅲ度烫伤模型。模型建立后,治疗组大鼠尾静脉注射1 mL VEGF抗体稀释液,对照组大鼠尾静脉注射1 mL生理盐水,然后分别检测两组大鼠烫伤后2、8、24、48、72 h的血清VEGF含量。结果烫伤后2、8、24、48、72 h,治疗组大鼠血清VEGF含量分别为(8.82±0.51)pg/mL、(16.79±7.01)pg/mL、(22.79±5.09)pg/mL、(29.81±3.91)pg/mL及(30.68±5.45)pg/mL,明显低于对照组大鼠的(138.97±7.59)pg/mL、(159.47±2.75)pg/mL、(171.43±15.23)pg/mL、(173.03±12.43)pg/mL及(189.23±6.41)pg/mL,两组对比,P均<0.01,差异具有统计学意义。结论烫伤初期大鼠体内注射VEGF抗体,可明显降低烫伤大鼠血清中VEGF的表达水平,从而降低血管通透性,减少液体渗出,由此推测,VEGF抗体可防止烧伤休克的发生与发展,值得进一步深入研究探讨。更多还原

【Abstract】 Objective To investigate the effect of VEGF antibody on shock in scalded rats.Methods Eightyeight rats were randomly selected as the study subjects,of which 8 rats were set as the pre-treatment group and the other 80 rats were evenly divided into the treatment group(n=40) and the control group(n=40) according to the random number table.Models of third-degree scalds were made in the treatment group and control group,followed by the tail vein injection of 1 mL VEGF antibody diluent in the treatment group and the tail vein injection of 1 mL normal saline.The serum VEGF levels of the scalded rats in the two groups were monitored at 2,8,24,48 and 72 h post injury respectively.Results The serum VEGF levels of rats in the treatment group at 2,8,24,48 and 72 h post injury were(8.82±0.51) pg/mL,(16.79±7.01) pg/mL,(22.79±5.09) pg/mL,(29.81±3.91) pg/mL and(30.68±5.45) pg/mL respectively,significantly being lower than the corresponding(138.97±7.59)pg/mL,(159.47±2.75) pg/mL,(171.43±5.23) pg/mL,(173.03±12.43)pg/mL and(189.23±6.41) pg/mL in the control group,and the comparison between them showed statistically significant difference(P<0.01).Conclusion The early-stage application of VEGF antibody injection into the scalded rats can remarkably decrease the expression level of VEGF in scalded rat serum,and further reduce the vascular permeability and the exudation,and it is thus speculated that VEGF antibody may prevent the development and progress of burn shock,which should be further investigated in future studies.更多还原