A two-dimensional gel electrophoresis(2-DE) system for the separation of myofibrillar proteins from silver carp was established and optimized in this study. The key factors of isoelectric focusing(IEF) and the p H gradient of immobilized p H gradient(IPG) strip were selected as the main factors affecting 2-DE in this paper. We loaded 120 μg of protein onto the 17 cm p H 4-7 IPG strip, and the IEF program III was as follows: 50 V for 15 h, three-stage desalting involving 250 V for 2 h, 1 000 V for 2.5 h and ...
引文格式:仪淑敏,李睿智,陈杨,等.白鲢鱼肌原纤维蛋白双向电泳分析体系的建立[J].食品科学,2017,38(1):41-46.DOI:10.7506/spkx1002-6630-201701007.http://www.spkx.net.cnYI Shumin,LI Ruizhi,CHEN Yang,et al.Establishment of two-dimensional gel electrophoresis system
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