【摘要】 以蛋白质和多酚的吸附率及多糖的回收率为考察指标,比较D-101、AB-8、X-5、ADS-7、ADS-17、DM-130等6种大孔吸附树脂对枇杷叶粗多糖的纯化效果,筛选出最佳树脂并研究其优化工艺,同时采用FRAP法和DPPH法测定纯化前后多糖抗氧化活性。实验结果最佳树脂为ADS-7,最佳工艺为:上样流速1.2 BV/h,p H为13,多糖浓度24.59 mg/m L,上样量为5 BV,回收流出液,并以体积分数10%乙醇洗脱回收多糖。多糖回收率达到85%,纯度由40.4%提高到94.6%,纯化倍数2.34倍。枇杷叶多糖DPPH自由基EC50从纯化前的4.21 mg/g降低到1.64 mg/g。结论:大孔树脂吸附法可用于纯化枇杷叶多糖,纯化后多糖自由基清除能力也得到提高。更多还原

【Abstract】 To study the process of purification of loquat polysaccharides by macroporous resin,the effect of deproteinization and polyphenol removal were compared among 6 kinds of macroporous resins,including D-101,AB-8,X-5,ADS-7,ADS-17,DM-130. The best macroporous resin was selected based on the adsorption rate of the protein and polyphenol,and the recovery rate of polysaccharide.The optimization process of loquat polysaccharide purification by the screening resin was studied through static and dynamic adsorption experiments.The antioxidant activity of polysaccharides before and after purified was determined by the method of FRAP and DPPH free radical scavenging.Compared with other 5 resin,ADS-7 resin was the most suitable one for preliminary purification of loquat polysaccharide.At room temperature( 25 ℃),the optimization process was as follows: the velocity was 1.2 BV/h,p H was 13,polysaccharide concentration was 24.59 mg/m L,sample volume was 5 BV,eluted by 10%ethanol to recovery of polysaccharide.Based on the optimization process,85% polysaccharide could be recovered,the content of polysaccharides was up to 94.6% from 40.4%,and purification multiple was 2.34 times.The EC50 of DPPH free radical of loquat polysaccharides after purified was down to 1.64 mg/g from 4.21 mg/g. Conclusion: the macroprous resin can be used to purify the loquat polysaccharides,and the purified polysaccharides had higher scavenging active of free radical than the crude polysaccharides.更多还原