【摘要】 为了研究板栗壳乙酸乙酯提取物体外活性,并分析其活性成分,依次采用DPPH法、ABTS法测定板栗壳乙酸乙酯提取物的抗氧化活性;PNPG法测定其体外抑制α-葡萄糖苷酶活性;超高效液相色谱—质谱联用技术(UPLC—MS/MS)对其成分进行分析。结果表明:板栗壳乙酸乙酯提取物具有较强的清除DPPH自由基、ABTS自由基的能力且可显著地抑制α-葡萄糖苷酶的活性,其有效成分主要为脂溶性黄酮类化合物。更多还原

【Abstract】 Investigated and analyzed the activity of ethyl acetate extract of chestnut shell and the components by UPLC—MS/MS.Antioxidant activities were carried out by DPPH radical scavenging assays and ABTS radical scavenging assays.The inhibitory activity of alpha glycosidase enzymes was measured by PNPG method.Qualitative analysis was performed by UPLC—MS/MS for analysis of major active components in ethyl acetate extracts.It determined that ethyl acetate extracts of Chestnut shell possessed remarkable DPPH radical scavenging activity,ABTS radical scavenging activity and high inhibitory activity of alpha glycosidase enzymes owing to the fatsoluble flavonoids are the main active components.更多还原