【摘要】 乳清蛋白是马乳中主要的蛋白质,不同物种间蛋白质组成存在差异。该研究利用反相高效液相色谱(RP-HPLC)对马乳清中的蛋白进行分离和定量分析,利用基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)对主要分离组分进行鉴定。结果表明,马乳乳清蛋白中主要组分为LYS,α-lg和β-lg。利用建立的色谱分析方法对乳清蛋白定量测定结果显示,LYS,α-lg和β-lg在(0.1~1.0)(R~2=0.999 3)、(0.2~0.8)(R~2=0.996 9)和(0.2~1.0)g/L(R~2=0.990 3)线性关系良好。该方法的稳定性、精密度、重现性的相对标准偏差(RSD)均小于5%,加标回收率分别为:88.05%~96.26%,86.03%~95.70%和91.79%~100.97%,能够满足实际检测的需要。测得马乳乳清中的LYS,α-lg和β-lg,含量为别为(3.38±0.32)、(2.64±0.37)及(0.52±0.06)g/L。更多还原

【Abstract】 Whey protein are the main protein in mare milk,but are different in protein composition among different species. In this paper,reserved-phase high-performance liquid chromatography( RP-HPLC) was used to isolate and quantify proteins in mare whey,the main components were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry( MALDI-TOF-MS). The result showed that LYS,α-Ig and β-Ig are the primary components in mare whey proteins. The linear relation of LYS,α-Ig and β-Ig are in the range of 0. 1-1. 0( R~2=0. 999 3) 0. 2-0. 8( R~2= 0. 996 9) and 0. 2-1. 0 μg/L( R~2= 0. 990 3),respectively. The relative standard deviations( RSDs) value of precision and reproducibility tests were all less than 5%,and the spike recovery was in the range of 88. 05%-96. 26%,86. 03%-95. 70% and 91. 79%-100. 97%,respectively. The concentration of LYS,α-lg and β-lg in mare milk were( 0. 52 ± 0. 06) 、( 3. 38 ± 0. 32) and( 2. 64 ± 0. 37) μg/L,respectively.更多还原