本研究旨在探索C型利钠肽前体蛋白基因(natriuretic peptide precursor C,nppc)在斑马鱼胚胎期的表达及其在血管发育过程中的功能。利用胚胎整体原位杂交技术检测nppc基因在斑马鱼胚胎期的表达。同时使用转基因斑马鱼系Tg(flk1:GFP)和Tg(fli1a:n GFP),显微注射nppc特异性吗啉基反义寡核苷酸(morpholino)和nppc m RNA调控nppc基因表达,利用激光共聚焦显微镜观察分析斑马鱼节间血管(intersegment vessel,ISV)表型,并统计内皮细胞数目。结果显示,在受精后24 h和48 h,nppc基因在斑马鱼脑、心脏、血管系统中都有表达。下调nppc基因表达导致ISV发育缺陷,ISV内皮细胞数目减少。以上结果表明下调nppc基因表达可能通过抑制内皮细胞增殖和内皮细胞迁移调控斑马鱼胚胎血管发育。
【英文摘要】
This study aimed to investigate the expression of the natriuretic peptide precursor C coding gene nppc and its role in angiogenesis during embryonic period of the zebrafish. Whole mount in situ hybridization was performed to detect the expression pattern of nppc. nppc specific morpholino and nppc m RNA were injected respectively into the one-cell stage embryo to specifically knock-down and rescue the expression of nppc in Tg(flk1:GFP) and Tg(fli1a:n GFP) transgenic lines. The morphology and endothelial cell...
【基金】
supported by grants from the National Natural Science Foundation of China(No.31370824);
the Special Support Plan of Excellent Young Talents,Guangdong Province,China(No.[2015]8);
Initial Program of Excellent Young Teachers in Colleges and Universities in Guangdong Province,China(No.[2016]6:YQ2015081)
【更新日期】
2017-03-16
【分类号】
R346
【正文快照】
This work was supported by grants from the National Natural Science Foundation of China(No.31370824),the Special SupportPlan of Excellent Young Talents,Guangdong Province,China(No.[2015]8)and Initial Program of Excellent Young Teachers in Col-leges and Un
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