【摘要】 本研究通过比较三种不同方法对霉菌毒素检测结果的差异,旨在为检测霉菌毒素提供科学依据。本实验采用酶联免疫(ELISA)试剂盒、超高效液相色谱(UHPLC)和液相质谱质谱联用(HPLC-MS/MS)三种检测方法,对仔猪配合饲料中黄曲霉毒素B1(AFB1)、玉米赤霉烯酮(ZEA)、呕吐毒素(DON)含量进行检测。ELISA法检测AFB1、ZEA和DON时,超标率与UHPLC法和HPLC-MS/MS法接近;但ELISA法检测AFB1结果为限量值为80%以上时,采用UHPLC法的检测结果显著高于HPLC-MS/MS法(P<0.05),其他所有样品的AFB1、ZEA和DON两种仪器检测方法差异不显著(P>0.05)。ELISA可作为霉菌毒素检测的快速初筛法;对ELISA结果进行确认时,低含量AFB1样品更宜采用HPLC-MS/MS法,其余样品采用UHPLC法检测更经济;对DON和ZEA的ELISA结果确认时,也可采用UHPLC法。更多还原

【Abstract】 The study was conducted to investigate the difference of three different methods to detect concentrations of mycotoxins,aims to give references about mycotoxins detection.A total of 33 piglet diets were selected to detect mycotoxins aflatoxins B1(AFB1),zearalenone(ZEA)and deoxynivalenol(DON) concentrations with different methods including ELISA,ultra performance liquid chromatography(UHPLC)and liquid chromatograph mass spectrometer mass spectrometer(HPLCMS/MS).The over standard rate of ELISA method on detection of AFB1,ZEA and DON was same with UHPLC and HPLCMS/MS methods,and the ELISA could be the preliminary screening method.When the concentration of AFB1 in piglet feed was 80% higher than limited value with the method of ELISA,the UHPLC detected AFB1 concentration was higher than that of HPLC-MS/MS(P < 0.05).While there were no significant difference in UHPLC and HPLC-MS/MS on concentrations of AFB1,ZEA and DON on other samples(P > 0.05).It was indicated that ELISA could be the preliminary screening method to detect the mycotoxins concentrations.HPLC-MS/MS could be used to verify the results of ELISA method with lower AFB1 concentration,while others were verified by UHPLC method.UHPLC could be used to verify the results of ELISA on concentration of DON and ZEA.更多还原