【摘要】 目的研究福建亚种猕猴的遗传多样性水平,为猕猴遗传质量监测方法提供基础资料。方法采用20个微卫星DNA(SSR)标记技术,经基因组DNA提取、PCR扩增、非变性聚丙烯酰胺凝胶电泳等,对28只福建亚种猕猴遗传多样性进行分析,用POPGENE 1.32等软件统计分析。结果福建亚种猕猴20个微卫星位点共检测到等位基因200个,观察等位基因数(Na)为10.0000±3.1119;有效等位基因数(Ne)为6.8438±2.4905;期望杂合度(He)为0.8509±0.0564;观察杂合度(Ho)为0.4607±0.1247;香隆信息指数(I)为2.0166±0.3463;多态信息含量(PIC)为0.8154±0.0665;显示检测的20个微卫星DNA位点均存在高度的遗传多态性,其中有17个位点偏离HardyWeinberg平衡(P<0.01)。结论有效地分析了福建亚种猕猴群体的遗传多态性,为今后建立福建亚种猕猴遗传质量监测方法提供理论依据。更多还原

【Abstract】 Objective To analyze the genetic diversity of Macaca mulatta littoralis and providing basic data for genetic quality monitoring methods of the population. Methods Twenty-eight Macaca mulatta littoralis were genotyped by using 20 microsatellite DNA markers, DNA was extracted from blood and amplified by PCR, and PCR products were analyzed by non-modified polyacrylamide gel electrophoresis.Every microsatellite locus was calculated by software POPGENE 1.32 and Excel. Results All 20 microsatellite loci were highly polymorphic, 200 alleles were found in the population.The number of alleles(Na), effective number of alleles(Ne), expected heterozygosity(He), observed heterozygosity(Ho),Shannon’s information index(I) and polymorphism information content(PIC) were ranged from 5 to 16,3.7333 to 11.6148, 0.7455 to 0.9305, 0.3214 to 0.7500, 1.3998 to 2.5963, and 0.6862 to 0.9074, with an average of 10,6.8438,0.8509,0.4607,2.0166 and 0.8154, respectively. All of 17 loci showed significant deviations from Hardy-Weinberg equilibrium. Conclusions There are high genetic diversity in Macaca mulatta littoralis of this colony. The experiments can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci.更多还原