【摘要】 目的:比较3种不同树脂水门汀对人牙龈成纤维细胞(human gingival fibroblasts,HGFs)的体外细胞毒性。方法:采用CCK-8法检测3种树脂水门汀(Panavia F、Rely XTM Unicem和Multilink Speed)48 h不同浓度(50%和100%)浸提液培养HGFs(ATCC,美国)24 h、72 h和120 h后的细胞相对增殖率(relative growth rate,RGR),判断其细胞毒性。采用SPSS 20.0软件包分析不同树脂水门汀对HGFs的RGR是否存在显著影响。结果:3种树脂水门汀不同浓度(50%和100%)浸提液培养HGFs的RGR为60.25%~100.02%,细胞毒性分级为0~2级,各组之间差异无显著性(P>0.05);固化过程中未涂布防氧化剂的Panavia F浸提液培养HGFs的RGR为10.67%~66.78%,细胞毒性分级为2~4级,与其他实验组相比,有显著差异(P<0.05)。结论:3种树脂水门汀对体外培养的HGFs无细胞毒性,固化过程中未涂布防氧化剂的Panavia F对体外培养的HGFs有中度细胞毒性。更多还原

【Abstract】 PURPOSE: To study the cytotoxicity of 3 resin cements to human gingival fibroblasts(HGFs). METHODS:Three resin cements(Panavia F, Rely XTMUnicem and Multilink Speed) test samples were immersed and incubated in the culture medium for 48 h at 37℃. Cultured HGFs were exposed to two concentrations(50% and 100%) of material elutes for24 h, 72 h and 120 h. The proliferation rate was evaluated using CCK-8 assay. The data were statistically analyzed by oneway analysis of variance using SPSS 20.0 software package. RESULTS: Relative growth rate(RGR) of all experimental groups ranged from 10.67% to 100.02%, the cytotoxicity grade of all groups was 0 to 4. There was no significant difference in the RGR among 3 resin cements, but the experimental group of Panavia F(uncovered with antioxidant) showed significantly lower RGR than other experimental groups. CONCLUSIONS: Panavia F, Rely XTMUnicem and Multilink Speed exhibit no cytotoxicity to HGFs, Panavia F(uncovered with antioxidant) shows moderate cytotoxicity.更多还原