【摘要】 目的观察脓毒症患者外周血内皮细胞蛋白C受体(EPCR)基因rs867186A/G位点多态性。方法 64例脓毒症患者(其中男38例、女26例)为脓毒症组,113例健康体检者为对照组。采集观察组、对照组5 mL外周静脉血,检测两组外周血EPCR基因rs867186A/G位点的多态性,采用ELISA法检测两组血浆可溶型EPCR(sEPCR)。分析EPCR基因rs867186A/G位点多态性对脓毒症患者血浆sEPCR表达及病情严重程度的影响结果脓毒症组AA、AG、GG三种基因型和A、G等位基因的分布频率分别为81.2%、17.2%、1.6%、89.8%、10.2%,对照组分别为83.2%、16.8%、0、91.6%、8.4%,两组间EPCR基因型及等位基因频率间差异均无统计学意义(P均>0.05)。EPCR基因rs867186A/G位点携带等位基因A的男性较携带相同等位基因的女性患脓毒症的风险高(OR=1.735;95%CI:1.063~2.833,P<0.05)。脓毒症组血浆sEPCR水平高于对照组但差异无统计学意义(P均>0.05)。不同rs867186A/G位点基因型脓毒症患者血浆sEPCR水平、病情严重程度间差异均无统计学意义(P均>0.05)。结论脓毒症患者外周血EPCR基因rs867186A/G位点上多为AA基因型或A等位基因。EPCR基因rs867186A/G位点多态性可能与脓毒症发病风险升高有关。更多还原

【Abstract】 Objective To observe the polymorphism of endothelial protein C receptor( EPCR) gene rs867186A/G in the peripheral blood of patients with sepsis. Methods Sixty-four patients with sepsis( sepsis group,male 38,female 26)and 113 healthy subjects( control group) were selected. We collected 5 mL peripheral blood samples from both the observation group and the control group. The polymorphisms of EPCR gene rs867186A/G in the peripheral blood were observed,and serum soluble EPCR( sEPCR) of two groups was measured by ELISA. Meanwhile,we investigated the effect of EPCR gene rs867186A/G polymorphisms on plasma sEPCR level and sepsis severity. Results The frequencies of AA,AG and GG genotypes and A,G alleles were 81. 2%,17. 2%,1. 6%,89. 8% and 10. 2% respectively,in sepsis group,versus83. 2%,16. 8%,0,91. 6% and 8. 4% in control group,and there was no significant difference in frequency distribution of the above genotypes and alleles of two groups( all P > 0. 05). Males carrying rs867186 A allele of EPCR gene were associated with a significantly increased risk of sepsis than women carrying the same alleles( OR = 1. 735,95% CI: 1. 063-2. 833,P < 0. 05). The plasma sEPCR level in the sepsis group was slightly higher than that in the control group but no significant difference was found( all P > 0. 05). There was no significant difference in plasma sEPCR level and severity of sepsis between patients with different rs867186A/G genotypes( all P > 0. 05). Conclusions The AA genotype or A allele is more frequently found in EPCR gene rs867186A/G in the peripheral blood of patients with sepsis. The polymorphism of EPCR gene rs867186A/G may be associated with the increased risk of sepsis.更多还原