【摘要】 建立了高效液相色谱法测定十全大补膏中芍药苷含量的方法。采用Kromasil C₁₈色谱柱（4.6 mm×250 mm,5μm）,以乙腈-水（17∶83,V/V）为流动相,柱温30℃,进样量10μL,检测波长230 nm,流速1.0 mL/min。结果表明,芍药苷在0.083 6⁰.627 0μg范围内线性关系良好（r=0.999 8）,加样回收率为98.98%¹00.47%,相对标准偏差为0.65%（n=6）。本方法专属性强、灵敏度高、重现性好、操作简便,适用于十全大补膏的质量控制。更多还原

【Abstract】 A HPLC method for the determination of paeoniflorin in tonic semifluid extract of ten ingredients was established.The HPLC analysis was performed on Kromasil C₁₈ column （4.6 mm × 250 mm,5 μm）,acetonitrile-water （1783,V/V）as the mobile phase,and the column temperature was 30 ℃,injection volume was 10 μL,the detective wavelength was at230 nm,at a flow rate of 1.0 m L/min.Results revealed that paeoniflorin at the content of 0.083 6 0.627 0 μg showed good linearity （r = 0.999 8）,the recovery rate was 98.98% 100.47%,RSD was 0.65% （n = 6）.This method is highly specific,sensitive,reproducible,and simple,which can be used for the quality control of this preparation.更多还原