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牦牛隐性乳房炎病原菌的分离及鉴定

Isolation and Identification of Yak Subclinical Mastitis Pathogenic Bacteria from Milk Samples

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【作者】 李小旭刘侃张小琴郝葆青

【Author】 Li Xiaoxu;Liu Kan;Zhang Xiaoqin;College of Life Science and Technology,Southwest University for Nationalities;Department of Chemistry,Chemical Engineering and Life Science,Aba Teachers College;

【机构】 西南民族大学生命科学与技术学院阿坝师范学院化学化工与生命科学系

【摘要】 牦牛隐性乳房炎被认为是高原藏族地区牦牛奶产量低下的主要原因之一。探索和研究牦牛隐性乳房炎致病菌特性及流行病学特征,可为牦牛隐性乳房炎防治提供一定的依据。本试验采集了红原地区不同牧群牦牛的80份奶样,由体细胞数、p H值测定初步筛选出了30份疑似牦牛乳房炎奶样,然后对其进行培养观察及生化检测,分离并鉴定出了牦牛隐性乳房炎病原菌。结果显示:80份奶样中有28份为牦牛隐性乳房炎奶样,检出率为35%;细菌学检测表明,28份奶样中金黄色葡萄球菌占10.71%,无乳链球菌占25%,乳房链球菌占32.14%,停乳链球菌占17.86%,大肠杆菌占14.29%。

【Abstract】 Yak subclinical mastitis is considered one of the primary causes for decreasing the milk yield of yak in Tibetan plateau.It has great practical significance that explore and research the characteristic of pathogenic bacterium and epidemiology characteristic, could provide certain basis for the prevention of yak subclinical mastitis at Hongyuan Tibetan area. In this study,80 milk samples were collected from three different herd in Hongyuan county, 30 milk samples were screened as suspected yak subclinical mastitis by milk somatic cell count and p H value. Afterwards,the suspected milk samples were cultivated for observation, biochemical identification, and other further testing. The results showed that, 28 milk samples were subclinical mastitis in the 80 milk samples,its detection rate was 35%. Bacteriological tests indicated that among the 80 milk samples,staphylococcus aureus was account for 10.71%,streptococcus agalactiae was account for 25%, streptococcus uberis was account for 32.14%, streptococcus dysgalactiae was account for17.86%,Escherichia coli was account for 14.29%.

  • 【文献出处】 四川畜牧兽医 ,Sichuan Animal & Veterinary Sciences , 编辑部邮箱 ,2017年03期
  • 【分类号】S858.23
  • 【被引频次】4
  • 【下载频次】69
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